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Neogen palcam agar base
Palcam Agar Base, supplied by Neogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/palcam+agar/agar+count+plate/pm41892577-253-138-142
Average 86 stars, based on 1 article reviews
palcam agar base - by Bioz Stars, 2026-10
86/100 stars

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Incubation:

Article Title: Novel Applications of Lactic Acid and Acetic Acid Bacteria Preparations in Shaping the Technological and Microbiological Quality of Ready-to-Cook Minced Pork.
Article Snippet: Samples were blended at 230 rpm using a Stomacher 400 instrument (IUL Instruments, Königswinter, Germany) for 2 min, followed by serial decimal dilutions and plating on selective media. .. The total viable count (TVC) was determined using Plate Count Agar (PCA) (Merck Life Science, Darmstadt, Germany), incubated at 30 ◦C for 48 h. Lactic acid bacteria (LAB) were enumerated on MRS agar (Neogen, Lansing, MI, USA) and incubated at 37 ◦C for 48 h. Escherichia coli was detected using Tryptone Bile X-Glucuronide (TBX) agar (Oxoid, Basingstoke, UK), incubated at 44 ◦C for 18–24 h. Enterobacteriaceae were plated on Violet Red Bile Glucose (VRBG) agar (Oxoid, Basingstoke, UK) and incubated at 37 ◦C for 24 h. Coagulasepositive Staphylococcus were detected using a Rabbit Plasma Fibrinogen (RPF) (Biokar Diagnostics, Allonne, France) medium incubated at 37 ◦C for 18–24 h. Salmonella spp. detection involved enrichment in Müller–Kauffman’s tetrathionate broth with novobiocin (Oxoid, Basingstoke, UK), followed by plating on XLD agar (Oxoid, Basingstoke, UK) and Rambach chromogenic medium (Merck KGaA, Darmstadt, Germany), incubated at 37 ◦C for 24 h. Listeria monocytogenes was enriched in Half Fraser and Fraser broths with the X211 supplement (Neogen, Lansing, MI, USA) followed by plating on Listeria chromogenic agar with ×10 and ×72 supplements (Neogen, Lansing, MI, USA) and Palcam agar (Neogen, Lansing, MI, USA), incubated at 37 ◦C for 24–48 h. Bacterial counts were calculated and expressed as log10 CFU/g. ..

Article Title: Prevalence of Listeria spp. in produce handling and processing facilities in the Pacific Northwest.
Article Snippet: Sample swabs were massaged by hand or with a stomacher and 136 incubated at 30°C for 24 h. Following incubation, samples were mixed with 100 μl 137 transferred to 10 ml of Fraser broth (FB, Neogen, Lansing, MI, USA) and incubated at 35°C 138 for 24-48 h with shaking at 200 rpm. .. Aliquots (10 μl) of the incubated DFB and/or FB were 139 spread plated onto both a Harlequin Listeria agar according to the formulation of Ottaviani 140 and Agosti (ALOA, Neogen, Lansing, MI, USA), and PALCAM agar (Neogen, Lansing, MI, 141 USA); incubated at 35°C for 24-48 h. ALOA and PALCAM plates were evaluated for the 142 presence of typical Listeria spp. colonies. .. For each sample, up to 20 colonies displaying 143 morphology typical for Listeria spp. were selected and transferred by stabbing to trypticase 144 soy agar (TSA) + 5% defibrinated horse blood (HBA; Hardy Diagnostics, Santa Maria, CA, 145 USA).

Bacteria:

Article Title: Novel Applications of Lactic Acid and Acetic Acid Bacteria Preparations in Shaping the Technological and Microbiological Quality of Ready-to-Cook Minced Pork.
Article Snippet: Samples were blended at 230 rpm using a Stomacher 400 instrument (IUL Instruments, Königswinter, Germany) for 2 min, followed by serial decimal dilutions and plating on selective media. .. The total viable count (TVC) was determined using Plate Count Agar (PCA) (Merck Life Science, Darmstadt, Germany), incubated at 30 ◦C for 48 h. Lactic acid bacteria (LAB) were enumerated on MRS agar (Neogen, Lansing, MI, USA) and incubated at 37 ◦C for 48 h. Escherichia coli was detected using Tryptone Bile X-Glucuronide (TBX) agar (Oxoid, Basingstoke, UK), incubated at 44 ◦C for 18–24 h. Enterobacteriaceae were plated on Violet Red Bile Glucose (VRBG) agar (Oxoid, Basingstoke, UK) and incubated at 37 ◦C for 24 h. Coagulasepositive Staphylococcus were detected using a Rabbit Plasma Fibrinogen (RPF) (Biokar Diagnostics, Allonne, France) medium incubated at 37 ◦C for 18–24 h. Salmonella spp. detection involved enrichment in Müller–Kauffman’s tetrathionate broth with novobiocin (Oxoid, Basingstoke, UK), followed by plating on XLD agar (Oxoid, Basingstoke, UK) and Rambach chromogenic medium (Merck KGaA, Darmstadt, Germany), incubated at 37 ◦C for 24 h. Listeria monocytogenes was enriched in Half Fraser and Fraser broths with the X211 supplement (Neogen, Lansing, MI, USA) followed by plating on Listeria chromogenic agar with ×10 and ×72 supplements (Neogen, Lansing, MI, USA) and Palcam agar (Neogen, Lansing, MI, USA), incubated at 37 ◦C for 24–48 h. Bacterial counts were calculated and expressed as log10 CFU/g. ..

Clinical Proteomics:

Article Title: Novel Applications of Lactic Acid and Acetic Acid Bacteria Preparations in Shaping the Technological and Microbiological Quality of Ready-to-Cook Minced Pork.
Article Snippet: Samples were blended at 230 rpm using a Stomacher 400 instrument (IUL Instruments, Königswinter, Germany) for 2 min, followed by serial decimal dilutions and plating on selective media. .. The total viable count (TVC) was determined using Plate Count Agar (PCA) (Merck Life Science, Darmstadt, Germany), incubated at 30 ◦C for 48 h. Lactic acid bacteria (LAB) were enumerated on MRS agar (Neogen, Lansing, MI, USA) and incubated at 37 ◦C for 48 h. Escherichia coli was detected using Tryptone Bile X-Glucuronide (TBX) agar (Oxoid, Basingstoke, UK), incubated at 44 ◦C for 18–24 h. Enterobacteriaceae were plated on Violet Red Bile Glucose (VRBG) agar (Oxoid, Basingstoke, UK) and incubated at 37 ◦C for 24 h. Coagulasepositive Staphylococcus were detected using a Rabbit Plasma Fibrinogen (RPF) (Biokar Diagnostics, Allonne, France) medium incubated at 37 ◦C for 18–24 h. Salmonella spp. detection involved enrichment in Müller–Kauffman’s tetrathionate broth with novobiocin (Oxoid, Basingstoke, UK), followed by plating on XLD agar (Oxoid, Basingstoke, UK) and Rambach chromogenic medium (Merck KGaA, Darmstadt, Germany), incubated at 37 ◦C for 24 h. Listeria monocytogenes was enriched in Half Fraser and Fraser broths with the X211 supplement (Neogen, Lansing, MI, USA) followed by plating on Listeria chromogenic agar with ×10 and ×72 supplements (Neogen, Lansing, MI, USA) and Palcam agar (Neogen, Lansing, MI, USA), incubated at 37 ◦C for 24–48 h. Bacterial counts were calculated and expressed as log10 CFU/g. ..

Sterility:

Article Title: Microbiome and Physicochemical Features Associated with Differential Listeria monocytogenes Growth in Soft, Surface-Ripened Cheeses.
Article Snippet: .. The homogenized samples were then diluted in sterile PBS before spread plating on PALCAM agar (Neogen Corp., Lansing, MI). ..

Homogenization:

Article Title: The effect of sodium reduction with and without potassium chloride on the survival of Listeria monocytogenes in Cheddar cheese.
Article Snippet: On each sampling occasion, 2 sample bags per treatment were removed from storage and homogenized at approximately 230 rpm (Stomacher Lab Blender 400; Seward Laboratory Systems Inc., Port Saint Lucie, FL) with 90 mL of buffered peptone water Journal of Dairy Science Vol. .. 96 No. 10, 2013 (Neogen Corp.) for 2 min. After homogenization, serial dilutions (2 dilutions per sample) were performed and the samples were spread plated (2 plates per dilution, 0.1 mL spread plated) on PALCAM agar (Neogen Corp.). .. When the counts of L. monocytogenes were below the limit of detection (15 cfu/g), cheese samples were enriched with UVM modified Listeria enrichment broth (Neogen Corp.) for 24 h at 37°C.

Cell Culture:

Article Title: Effect of Lactose Monolaurate on Pathogenic and Nonpathogenic Bacteria
Article Snippet: .. The Listeria strains were initially grown and plated on Palcam agar with supplements (polymyxin B, acriflavine, and ceftazidime, all from Neogen, Lansing, MI) to confirm purity, and individual isolates were used for the remainder of the study and cultured in brain heart infusion (BHI) medium. table ft1 table-wrap mode=article t1 caption a4 Growth media and microorganisms used in this study LML was synthesized as described previously ( 12 ) and purified to 98% via differential solubility of the reactants (lactose and vinyl laurate) from the product in 50% ethanol-water. ..

Synthesized:

Article Title: Effect of Lactose Monolaurate on Pathogenic and Nonpathogenic Bacteria
Article Snippet: .. The Listeria strains were initially grown and plated on Palcam agar with supplements (polymyxin B, acriflavine, and ceftazidime, all from Neogen, Lansing, MI) to confirm purity, and individual isolates were used for the remainder of the study and cultured in brain heart infusion (BHI) medium. table ft1 table-wrap mode=article t1 caption a4 Growth media and microorganisms used in this study LML was synthesized as described previously ( 12 ) and purified to 98% via differential solubility of the reactants (lactose and vinyl laurate) from the product in 50% ethanol-water. ..

Purification:

Article Title: Effect of Lactose Monolaurate on Pathogenic and Nonpathogenic Bacteria
Article Snippet: .. The Listeria strains were initially grown and plated on Palcam agar with supplements (polymyxin B, acriflavine, and ceftazidime, all from Neogen, Lansing, MI) to confirm purity, and individual isolates were used for the remainder of the study and cultured in brain heart infusion (BHI) medium. table ft1 table-wrap mode=article t1 caption a4 Growth media and microorganisms used in this study LML was synthesized as described previously ( 12 ) and purified to 98% via differential solubility of the reactants (lactose and vinyl laurate) from the product in 50% ethanol-water. ..

Solubility:

Article Title: Effect of Lactose Monolaurate on Pathogenic and Nonpathogenic Bacteria
Article Snippet: .. The Listeria strains were initially grown and plated on Palcam agar with supplements (polymyxin B, acriflavine, and ceftazidime, all from Neogen, Lansing, MI) to confirm purity, and individual isolates were used for the remainder of the study and cultured in brain heart infusion (BHI) medium. table ft1 table-wrap mode=article t1 caption a4 Growth media and microorganisms used in this study LML was synthesized as described previously ( 12 ) and purified to 98% via differential solubility of the reactants (lactose and vinyl laurate) from the product in 50% ethanol-water. ..

Formulation:

Article Title: Prevalence of Listeria spp. in produce handling and processing facilities in the Pacific Northwest.
Article Snippet: Sample swabs were massaged by hand or with a stomacher and 136 incubated at 30°C for 24 h. Following incubation, samples were mixed with 100 μl 137 transferred to 10 ml of Fraser broth (FB, Neogen, Lansing, MI, USA) and incubated at 35°C 138 for 24-48 h with shaking at 200 rpm. .. Aliquots (10 μl) of the incubated DFB and/or FB were 139 spread plated onto both a Harlequin Listeria agar according to the formulation of Ottaviani 140 and Agosti (ALOA, Neogen, Lansing, MI, USA), and PALCAM agar (Neogen, Lansing, MI, 141 USA); incubated at 35°C for 24-48 h. ALOA and PALCAM plates were evaluated for the 142 presence of typical Listeria spp. colonies. .. For each sample, up to 20 colonies displaying 143 morphology typical for Listeria spp. were selected and transferred by stabbing to trypticase 144 soy agar (TSA) + 5% defibrinated horse blood (HBA; Hardy Diagnostics, Santa Maria, CA, 145 USA).



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